Molecular Oncology
Volume 1, Issue 1 , Pages 72-83 , June 2007

Mice thrive without Cdk4 and Cdk2

  • Cédric Barrière

      Affiliations

    • Molecular Oncology, Centro Nacional de Investigaciones Oncológicas, E-28029 Madrid, Spain
    • EA2406 University of Bordeaux 2, F-33076 Bordeaux, France
    • These authors contributed equally to this work.
    • ,
  • David Santamaría

      Affiliations

    • Molecular Oncology, Centro Nacional de Investigaciones Oncológicas, E-28029 Madrid, Spain
    • These authors contributed equally to this work.
    • ,
  • Antonio Cerqueira

      Affiliations

    • Molecular Oncology, Centro Nacional de Investigaciones Oncológicas, E-28029 Madrid, Spain
    • ,
  • Javier Galán

      Affiliations

    • Molecular Oncology, Centro Nacional de Investigaciones Oncológicas, E-28029 Madrid, Spain
    • ,
  • Alberto Martín

      Affiliations

    • Molecular Oncology, Centro Nacional de Investigaciones Oncológicas, E-28029 Madrid, Spain
    • ,
  • Sagrario Ortega

      Affiliations

    • Biotechnology Program, Centro Nacional de Investigaciones Oncológicas, E-28029 Madrid, Spain
    • ,
  • Marcos Malumbres

      Affiliations

    • Molecular Oncology, Centro Nacional de Investigaciones Oncológicas, E-28029 Madrid, Spain
    • ,
  • Pierre Dubus

      Affiliations

    • EA2406 University of Bordeaux 2, F-33076 Bordeaux, France
    • ,
  • Mariano Barbacid

      Affiliations

    • Molecular Oncology, Centro Nacional de Investigaciones Oncológicas, E-28029 Madrid, Spain
    • Corresponding Author InformationCorresponding author. Centro Nacional de Investigaciones Oncológicas (CNIO), Melchor Fernández Almagro 3, E-28029 Madrid, Spain. Tel.: +34 91 2246900; Fax: +34 91 7328033.

Received 22 December 2006 ,Revised 15 February 2007 ,Accepted 15 February 2007.

Title About Type File Size
Supplemental Figure S1

Characterization of hematopoietic compartments in adult mice lacking Cdk4 and Cdk2. (A) Colony formation assay of bone marrow cells in methylcellulose. Bars represent number of colony-forming units for granulocytes (CFU-G), macrophages (CFU-M), mixed granulocytes/macrophages (CFU-GMM) and erythrocytes (BFU-E), obtained from seeding 5×104 cells. (B–D) Flow cytometry analysis of the major hematopoietic populations in adult organs. Single cell suspensions were immunostained with antibodies against the indicated surface markers and the relative percentages of specific populations were quantified by cytometry. Open boxes represent samples obtained from 4OHT-treated Cdk4+/+;Cdk2+/+;RERTert/ert control mice. Solid boxes represent samples obtained from treated Cdk4−/−;Cdk2Δ/Δ;RERTert/ert mice. Error bars: SD.

 Image 0 MB

Please note that add-on components may require plug-in applications.

PII: S1574-7891(07)00011-7

doi: 10.1016/j.molonc.2007.03.001

Molecular Oncology
Volume 1, Issue 1 , Pages 72-83 , June 2007

Article Tools

* Image Usage & Resolution