Molecular Oncology
Volume 1, Issue 2 , Pages 181-195 , September 2007

Phosphoprotein Keratin 23 accumulates in MSS but not MSI colon cancers in vivo and impacts viability and proliferation in vitro

  • Karin Birkenkamp-Demtroder

      Affiliations

    • Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital/Skejby, Brendstrupgaardsvej, DK-8200 Aarhus N, Denmark
    • Corresponding Author InformationCorresponding author: Molecular Diagnostic Laboratory, Center for Molecular Clinical Cancer Research CMCC, Department of Clinical Biochemistry, Aarhus University Hospital/Skejby, Brendstrupgaardsvej, DK-8200 Aarhus N, Denmark. Tel.: +45 8949 5119; fax: +45 8949 6018.
    • KBD and FM have contributed equally to this publication.
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    • ,
  • Francisco Mansilla

      Affiliations

    • Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital/Skejby, Brendstrupgaardsvej, DK-8200 Aarhus N, Denmark
    • KBD and FM have contributed equally to this publication.
    • ,
  • Flemming Brandt Sørensen

      Affiliations

    • Department of Pathology, Aarhus University Hospital, Nørrebrogade, DK-8000 Århus C, Denmark
    • ,
  • Mogens Kruhøffer

      Affiliations

    • Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital/Skejby, Brendstrupgaardsvej, DK-8200 Aarhus N, Denmark
    • ,
  • Teresa Cabezón

      Affiliations

    • Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen, Denmark
    • ,
  • Lise Lotte Christensen

      Affiliations

    • Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital/Skejby, Brendstrupgaardsvej, DK-8200 Aarhus N, Denmark
    • ,
  • Lauri A. Aaltonen

      Affiliations

    • Finnish Cancer Institute, Department of Medical Genetics, Biomedicum Helsinki, PO Box 63 (Haartmaninkatu 8), FIN-00014 University of Helsinki, Finland
    • ,
  • Hein W. Verspaget

      Affiliations

    • Department of Gastroenterology-Hepatology, Leiden University Medical Centre, PO Box 9600, NL-2300 RC Leiden, Netherlands
    • ,
  • Torben Falck Ørntoft

      Affiliations

    • Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital/Skejby, Brendstrupgaardsvej, DK-8200 Aarhus N, Denmark

Received 30 March 2007 ,Revised 15 May 2007 ,Accepted 21 May 2007.

References 

  1. Archer SY, Meng S, Shei A, Hodin RA. p21(WAF1) is required for butyrate-mediated growth inhibition of human colon cancer cells. Proc. Natl. Acad. Sci. U.S.A. 1998;95:6791–6796
  2. Benatti P, Gafa R, Barana D, Marino M, Scarselli A, Pedroni M, et al. Microsatellite instability and colorectal cancer prognosis. Clin. Cancer Res. 2005;11:8332–8340
  3. Birkenkamp-Demtroder K, Christensen LL, Olesen SH, Frederiksen CM, Laiho P, Aaltonen LA, et al. Gene expression in colorectal cancer. Cancer Res. 2002;62:4352–4363
  4. Birkenkamp-Demtroder K, Olesen SH, Sorensen FB, Laurberg S, Laiho P, Aaltonen LA, et al. Differential gene expression in colon cancer of the caecum versus the sigmoid and rectosigmoid. Gut. 2005;54:374–384
  5. Birkenkamp-Demtroder K, Wagner L, Sørensen FB, Bording Astrup L, Gartner W, Scherubl H, et al. Secretagogin is a novel marker for neuroendocrine differentiation. Neuroendocrinology. 2005;82:121–138
  6. Castedo M, Perfettini JL, Roumier T, Valent A, Raslova H, Yakushijin K, et al. Mitotic catastrophe constitutes a special case of apoptosis whose suppression entails aneuploidy. Oncogene. 2004;23:4362–4370
  7. Caulin C, Salvesen GS, Oshima RG. Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis. J. Cell Biol. 1997;138:1379–1394
  8. Celis JE, Ratz G, Basse B, Lauridsen JB, Celis A. High-resolution two-dimensional gel electrophoresis of proteins: isoelectric focusing and non-equilibrium pH gradient electrophoresis (NEPHGE). Cell Biol. Lab. Handbook. 1994;III:222–230
  9. de La Chapelle A. Microsatellite instability. N. Engl. J. Med. 2003;349:209–210
  10. Gradilone A, Gazzaniga P, Silvestri I, Gandini O, Trasatti L, Lauro S, et al. Detection of CK19, CK20 and EGFR mRNAs in peripheral blood of carcinoma patients: correlation with clinical stage of disease. Oncol. Rep. 2003;10:217–222
  11. Heinemeyer T, Wingender E, Reuter I, Hermjakob H, Kel AE, Kel OV, et al. Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL. Nucleic Acids Res. 1998;26:362–367
  12. Izawa I, Inagaki M. Regulatory mechanisms and functions of intermediate filaments: a study using site- and phosphorylation state-specific antibodies. Cancer Sci. 2006;97:167–174
  13. Katsumoto T, Inoue M, Naguro T, Kurimura T. Association of cytoskeletons with the Golgi apparatus: three-dimensional observation and computer-graphic reconstruction. J. Electron Microsc. (Tokyo). 1991;40:24–28
  14. Kim H, Nam SW, Rhee H, Shan LL, Ju KH, Hye KK, et al. Different gene expression profiles between microsatellite instability-high and microsatellite stable colorectal carcinomas. Oncogene. 2004;19(23):6218–6225
  15. Kirfel J, Magin TM, Reichelt J. Keratins: a structural scaffold with emerging functions. Cell Mol. Life Sci. 2003;60:56–71
  16. Kruhoffer M, Jensen JL, Laiho P, Dyrskjot L, Salovaara R, Arango D, et al. Gene expression signatures for colorectal cancer microsatellite status and HNPCC. Br. J. Cancer. 2005;20(92):2240–2248
  17. Ku NO, Omary MB. Effect of mutation and phosphorylation of type I keratins on their caspase-mediated degradation. J. Biol. Chem. 2001;20(276):26792–26798
  18. Lengauer C, Kinzler KW, Vogelstein B. Genetic instabilities in human cancers. Nature. 1998;396:643–649
  19. Levy FO, Oyen O, Sandberg M, Tasken K, Eskild W, Hansson V, et al. Molecular cloning, complementary deoxyribonucleic acid structure and predicted full-length amino acid sequence of the hormone-inducible regulatory subunit of 3′-5′-cyclic adenosine monophosphate-dependent protein kinase from human testis. Mol. Endocrinol. 1988;2:1364–1373
  20. Lothe RA, Peltomaki P, Meling GI, Aaltonen LA, Nystrom-Lahti M, Pylkkanen L, et al. Genomic instability in colorectal cancer: relationship to clinicopathological variables and family history. Cancer Res. 1993;53:5849–5852
  21. Moll R. Cytokeratins as markers of differentiation in the diagnosis of epithelial tumors. Subcell. Biochem. 1998;31:205–262
  22. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982;31:11–24
  23. Ogawa C, Iwatsuki H, Suda M, Sasaki K. Golgi-associated filament networks in duct epithelial cells of rabbit submandibular glands: immunohistochemical light and electron microscopic studies. Histochem. Cell Biol. 2002;118:35–40
  24. Olesen SH, Christensen LL, Sorensen FB, Cabezon T, Laurberg S, Orntoft TF, et al. Human FK506 binding protein 65 is associated with colorectal cancer. Mol. Cell Proteomics. 2005;4:534–544
  25. Omary MB, Ku NO, Tao GZ, Toivola DM, Liao J. ‘Heads and tails’ of intermediate filament phosphorylation: multiple sites and functional insights. Trends Biochem. Sci. 2006;31:383–394
  26. Oshima RG. Apoptosis and keratin intermediate filaments. Cell Death Differ. 2002;9:486–492
  27. Potter CS, Peterson RL, Barth JL, Pruett ND, Jacobs DF, Kern MJ, et al. Evidence that the satin hair mutant gene Foxq1 is among multiple and functionally diverse regulatory targets for Hoxc13 during hair follicle differentiation. J. Biol. Chem. 2006;281:29245–29255
  28. Rajagopalan H, Lengauer C. Aneuploidy and cancer. Nature. 2004;432:338–341
  29. Ribic CM, Sargent DJ, Moore MJ, Thibodeau SN, French AJ, Goldberg RM, et al. Tumor microsatellite-instability status as a predictor of benefit from fluorouracil-based adjuvant chemotherapy for colon cancer. N. Engl. J. Med. 2003;349:247–257
  30. Richon VM, Sandhoff TW, Rifkind RA, Marks PA. Histone deacetylase inhibitor selectively induces p21WAF1 expression and gene-associated histone acetylation. Proc. Natl. Acad. Sci. U.S.A. 2000;97:10014–10019
  31. Rogers MA, Winter H, Langbein L, Bleiler R, Schweizer J. The human type I keratin gene family: characterization of new hair follicle specific members and evaluation of the chromosome 17q21.2 gene domain. Differentiation. 2004;72:527–540
  32. Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, et al. New consensus nomenclature for mammalian keratins. J. Cell Biol. 2006;174:169–174
  33. Shibuya K, Mathers CD, Boschi-Pinto C, Lopez AD, Murray CJ. Global and regional estimates of cancer mortality and incidence by site: II. Results for the global burden of disease 2000. BMC. Cancer. 2002;2(37):37;Epub; 2002 Dec 26
  34. Stewart BW, Kleihues P. World Cancer Report. World Health Organization WHO Press; 2003;ISBN 13-9789283204114
  35. Stuhler K, Koper K, Pfeiffer K, Tagariello A, Souquet M, Schwarte-Waldhoff I, et al. Differential proteome analysis of colon carcinoma cell line SW480 after reconstitution of the tumour suppressor Smad4. Anal. Bioanal. Chem. 2006;386:1603–1612
  36. Toivola DM, Tao GZ, Habtezion A, Liao J, Omary MB. Cellular integrity plus: organelle-related and protein-targeting functions of intermediate filaments. Trends Cell Biol. 2005;15:608–617
  37. Wang Y, Jatkoe T, Zhang Y, Mutch MG, Talantov D, Jiang J, et al. Gene expression profiles and molecular markers to predict recurrence of Dukes’ B colon cancer. J. Clin. Oncol. 2004;22:1564–1571
  38. Wilson AJ, Byun DS, Popova N, Murray LB, L'Italien K, Sowa Y, et al. Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer. J. Biol. Chem. 2006;281:13548–13558
  39. Yano T, Tokui T, Nishi Y, Nishizawa K, Shibata M, Kikuchi K, et al. Phosphorylation of keratin intermediate filaments by protein kinase C, by calmodulin-dependent protein kinase and by cAMP-dependent protein kinase. Eur. J. Biochem. 1991;197:281–290
  40. Zhang JS, Wang L, Huang H, Nelson M, Smith DI. Keratin 23 (K23), a novel acidic keratin, is highly induced by histone deacetylase inhibitors during differentiation of pancreatic cancer cells. Genes Chromosomes Cancer. 2001;30:123–135
  41. Zhou Q, Cadrin M, Herrmann H, Chen CH, Chalkley RJ, Burlingame AL, et al. Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker. J. Biol. Chem. 2006;281:16453–16461

PII: S1574-7891(07)00035-X

doi: 10.1016/j.molonc.2007.05.005

Molecular Oncology
Volume 1, Issue 2 , Pages 181-195 , September 2007

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