Molecular Oncology
Volume 1, Issue 2 , Pages 196-204, September 2007

Green tea catechins suppress the DNA synthesis marker MCM7 in the TRAMP model of prostate cancer

  • Susan McCarthy

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • These authors contributed equally to this work.
    • ,
  • Andrea Caporali

      Affiliations

    • Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Parma, Via Volturno 39, 43100 Parma, Italy
    • These authors contributed equally to this work.
    • ,
  • Steve Enkemann

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • ,
  • Maurizio Scaltriti

      Affiliations

    • Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Parma, Via Volturno 39, 43100 Parma, Italy
    • ,
  • Steve Eschrich

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • ,
  • Pierpaola Davalli

      Affiliations

    • Department of Scienze Biomediche, University of Modena, Modena, Italy
    • ,
  • Arnaldo Corti

      Affiliations

    • Department of Scienze Biomediche, University of Modena, Modena, Italy
    • ,
  • Alice Lee

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • ,
  • Jimmy Sung

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • ,
  • Timothy J. Yeatman

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • Corresponding Author InformationCorresponding author at: Department of Surgery, H. Lee Moffitt Cancer Center, 12902 Magnolia Drive, Tampa, FL 33612, USA. Tel.: +1 813 979 7292; fax: +1 813 632 1433.
    • ,
  • Saverio Bettuzzi

      Affiliations

    • Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Parma, Via Volturno 39, 43100 Parma, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39 0521 903803; fax: +39 0521 903802.

Received 10 April 2007; received in revised form 29 May 2007; accepted 30 May 2007. published online 09 July 2007.

Abstract 

Green tea catechins (GTCs) exert chemopreventive effects in many cancer models. Several studies implicate the DNA synthesis marker minichromosome maintenance protein 7 (MCM7) in prostate cancer progression, growth and invasion; representing a novel therapeutic target. In this study, we investigated the effect of GTCs on MCM7 expression in the transgenic adenocarcinoma mouse prostate model (TRAMP). DNA microarray, immunohistochemistry and Western blot analysis showed that GTCs significantly suppressed MCM7 in the TRAMP mice treated with GTCs. Our study indicates that the cellular DNA replication factor MCM7 is involved in prostate cancer (CaP) and MCM7 gene expression was reduced by GTCs. Together, these results suggest a possible role of GTCs in CaP chemoprevention in which MCM7 plays a critical role.

Keywords: Chemoprevention, Green tea catechins, Prostate cancer, Transgenic adenocarcinoma mouse prostate model, Minichromosome maintenance protein 7

 

PII: S1574-7891(07)00039-7

doi:10.1016/j.molonc.2007.05.007

Molecular Oncology
Volume 1, Issue 2 , Pages 196-204, September 2007

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