Molecular Oncology
Volume 1, Issue 2 , Pages 196-204 , September 2007

Green tea catechins suppress the DNA synthesis marker MCM7 in the TRAMP model of prostate cancer

  • Susan McCarthy

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • These authors contributed equally to this work.
    • ,
  • Andrea Caporali

      Affiliations

    • Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Parma, Via Volturno 39, 43100 Parma, Italy
    • These authors contributed equally to this work.
    • ,
  • Steve Enkemann

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • ,
  • Maurizio Scaltriti

      Affiliations

    • Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Parma, Via Volturno 39, 43100 Parma, Italy
    • ,
  • Steve Eschrich

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • ,
  • Pierpaola Davalli

      Affiliations

    • Department of Scienze Biomediche, University of Modena, Modena, Italy
    • ,
  • Arnaldo Corti

      Affiliations

    • Department of Scienze Biomediche, University of Modena, Modena, Italy
    • ,
  • Alice Lee

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • ,
  • Jimmy Sung

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • ,
  • Timothy J. Yeatman

      Affiliations

    • Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA
    • Corresponding Author InformationCorresponding author at: Department of Surgery, H. Lee Moffitt Cancer Center, 12902 Magnolia Drive, Tampa, FL 33612, USA. Tel.: +1 813 979 7292; fax: +1 813 632 1433.
    • ,
  • Saverio Bettuzzi

      Affiliations

    • Dipartimento di Medicina Sperimentale, Sezione di Biochimica, Università di Parma, Via Volturno 39, 43100 Parma, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39 0521 903803; fax: +39 0521 903802.

Received 10 April 2007 ,Revised 29 May 2007 ,Accepted 30 May 2007.

  • Image Result

    Gene expression changes in the prostates of WT (non-transgenic), TRAMP water-fed and TRAMP mice treated with GTCs. Gene expression values were normalized, log2-transformed, and mean-centered to produc

    Gene expression changes in the prostates of WT (non-transgenic), TRAMP water-fed and TRAMP mice treated with GTCs. Gene expression values were normalized, log2-transformed, and mean-centered to produce relative values. Each column represents pooled samples from three different prostate glands (dorsolateral) taken from WT, TRAMP water-fed and TRAMP treated with GTCs at the 12, 17, and 24week time point. Some genes have multiple Affymetrix probe sets. Genes that are upregulated appear in red, and those that are downregulated appear in green. A color bar (bottom) relates to the intensity of the differences in gene expression.

  • Image Result
    Immunohistochemical staining for MCM7 protein in the dorsolateral prostate of WT and TRAMP mice. (A) WT (non transgenic) mouse at 24weeks of age (20×); (B) TRAMP water-fed mouse at 12weeks of age (20×

    Immunohistochemical staining for MCM7 protein in the dorsolateral prostate of WT and TRAMP mice. (A) WT (non transgenic) mouse at 24weeks of age (20×); (B) TRAMP water-fed mouse at 12weeks of age (20×); (C) TRAMP water-fed mouse at 17weeks of age (20×); (D) poorly differentiated prostate cancer in TRAMP water-fed mouse at 24weeks of age (20×); (E) prostate section of a GTCs-fed TRAMP mouse at 17weeks of age (20×); (F) prostate section of a GTCs-fed TRAMP mouse at 24weeks of age. MCM7 is not detectable in the normal monolayer of epithelial cells of the gland (arrow) but specifically expressed in the multilayer and high grade PIN lesions (40×).

  • Image Result
    Immunohistochemical staining for Ki-67 protein in the dorsolateral prostate of WT and TRAMP mice. (A) WT (non transgenic) mouse at 24weeks of age (20×); (B) TRAMP water-fed mouse at 12weeks of age (20

    Immunohistochemical staining for Ki-67 protein in the dorsolateral prostate of WT and TRAMP mice. (A) WT (non transgenic) mouse at 24weeks of age (20×); (B) TRAMP water-fed mouse at 12weeks of age (20×); (C) TRAMP water-fed mouse at 17weeks of age (20×); (D) poorly differentiated prostate cancer in TRAMP water-fed mouse at 24weeks of age (20×); (E) prostate section of a GTC-fed TRAMP mouse at 17weeks of age (20×); (F) prostate section of a GTC-fed TRAMP mouse at 24weeks of age (20×).

  • Image Result
    Percentage of (A) MCM7 and (B) Ki-67 positive cells in the dorsolateral prostates of WT (non-transgenic), TRAMP water-fed and TRAMP treated with GTCs at 12, 17 and 24weeks. The mean values with standa

    Percentage of (A) MCM7 and (B) Ki-67 positive cells in the dorsolateral prostates of WT (non-transgenic), TRAMP water-fed and TRAMP treated with GTCs at 12, 17 and 24weeks. The mean values with standard deviations were used to compare the percentage of positive cells for MCM7 and Ki-67 in each experimental group at the 12, 17 and 24week time point. Values represent mean±SD of five animals in each group. *P<0.05; ***P<0.001 compared with aged-matched TRAMP water-fed mice.

  • Image Result
    Western blot analysis of MCM7 protein levels in the dorsolateral prostates of 12, 17 and 24-week-old WT (non-transgenic), TRAMP water-fed and TRAMP treated mice with GTCs. Alpha-tubulin antibody was u

    Western blot analysis of MCM7 protein levels in the dorsolateral prostates of 12, 17 and 24-week-old WT (non-transgenic), TRAMP water-fed and TRAMP treated mice with GTCs. Alpha-tubulin antibody was used to show equal loading of the protein samples. Western blot is a representative of one (pooled samples three mice per group) of three experiments performed. (B) Histogram indicates the ratio between MCM7 and α-tubulin obtained by densitometry analysis of the immunoblot. Data are presented as the mean±SD of three animals in each group. ***P<0.001 compared to TRAMP treated with GTCs at 17 and 24weeks of age.

PII: S1574-7891(07)00039-7

doi: 10.1016/j.molonc.2007.05.007

Molecular Oncology
Volume 1, Issue 2 , Pages 196-204 , September 2007

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