Molecular Oncology
Volume 1, Issue 2 , Pages 226-231 , September 2007

Examination of gap junctional, intercellular communication by in situ electroporation on two co-planar indium-tin oxide electrodes

  • Aikaterini Anagnostopoulou

      Affiliations

    • Departments of Microbiology and Immunology and Pathology, Queen's University, Kingston, Ontario, K7L3N6, Canada
    • ,
  • Jun Cao

      Affiliations

    • Departments of Microbiology and Immunology and Pathology, Queen's University, Kingston, Ontario, K7L3N6, Canada
    • ,
  • Adina Vultur

      Affiliations

    • Departments of Microbiology and Immunology and Pathology, Queen's University, Kingston, Ontario, K7L3N6, Canada
    • ,
  • Kevin Firth

      Affiliations

    • Ask Sciences Products, Kingston, Ontario, K7L 3Z8, Canada
    • ,
  • Leda Raptis

      Affiliations

    • Departments of Microbiology and Immunology and Pathology, Queen's University, Kingston, Ontario, K7L3N6, Canada
    • Corresponding Author InformationCorresponding author. Tel.: +1 613 533 2462 (office/lab); +1 613 533 2450 (secretary); fax: +1 613 533 6796.

Received 13 May 2007 ,Revised 13 June 2007 ,Accepted 13 June 2007.

References 

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  2. Azarnia R, Russell TR. Cyclic AMP effects on cell-to-cell junctional membrane permeability during adipocytic differentiation of 3T3-L1 fibroblasts. J. Cell Biol. 1985;100:265–269
  3. Bahnson AB, Boggs SS. Addition of serum to electroporated cells enhances survival and transfection efficiency. Biochem. Biophys. Res. Commun. 1990;171:752–757
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  5. Brownell HL, Whitfield JF, Raptis L. Cellular Ras partly mediates gap junction closure by the polyoma virus middle Tumor antigen. Cancer Lett. 1996;103:99–106
  6. Brownell HL, Lydon N, Schaefer E, Roberts TM, Raptis L. Inhibition of epidermal growth factor-mediated ERK1/2 activation by in situ electroporation of nonpermeant [(alkylamino)methyl]acrylophenone derivatives. DNA Cell Biol. 1998;17:265–274
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  11. Raptis L, Brownell HL, Lu Y, Preston T, Narsimhan RP, Anderson S, et al. v-Ras and v-Raf block differentiation of transformable C3H10T1/2-derived preadipocytes at lower levels than required for neoplastic transformation. Exp. Cell Res. 1997;235:188–197
  12. Raptis L, Yang J, Brownell HL, Lai J, Preston T, Corbley MJ, et al. Rasleu61 blocks differentiation of transformable 3T3 L1 and C3HT1/2-derived preadipocytes in a dose- and time-dependent manner. Cell Growth Differ. 1997;8:11–21
  13. Raptis L, Brownell HL, Vultur AM, Ross G, Tremblay E, Elliott BE. Specific inhibition of growth factor-stimulated ERK1/2 activation in intact cells by electroporation of a Grb2-SH2 binding peptide. Cell Growth Differ. 2000;11:293–303
  14. Raptis L, Tomai E, Firth KL. Improved procedure for examination of gap junctional, intercellular communication by in situ electroporation on a partly conductive slide. Biotechniques. 2000;29:222–226
  15. Raptis L, Vultur A, Brownell HL, Firth KL. Dissecting pathways; in situ electroporation for the study of signal transduction and gap junctional communication. In:  Celis JE editors. Cell Biology: A Laboratory Handbook. San Diego, CA: Academic Press; 2006;p. 341–354
  16. Tomai E, Brownell HL, Tufescu T, Reid K, Raptis S, Campling BG, et al. A functional assay for intercellular, junctional communication in cultured human lung carcinoma cells. Lab. Invest. 1998;78:639–640
  17. Tomai E, Brownell HL, Tufescu T, Reid K, Raptis L. Gap junctional communication in lung carcinoma cells. Lung Cancer. 1999;23:223–231
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  19. Vultur A, Tomai E, Peebles K, Malkinson AM, Grammatikakis N, Forkert PG, et al. Gap junctional, intercellular communication in cells from urethane-induced tumors in A/J mice. DNA Cell Biol. 2003;22:33–40
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PII: S1574-7891(07)00041-5

doi: 10.1016/j.molonc.2007.06.002

Molecular Oncology
Volume 1, Issue 2 , Pages 226-231 , September 2007

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