Molecular Oncology
Volume 1, Issue 4 , Pages 431-439 , April 2008

The apoptosis linked gene ALG-2 is dysregulated in tumors of various origin and contributes to cancer cell viability

  • Jonas M. la Cour

      Affiliations

    • Copenhagen Biocenter, Department of Molecular Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark
    • ,
  • Berit R. Høj

      Affiliations

    • Copenhagen Biocenter, Department of Molecular Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark
    • ,
  • Jens Mollerup

      Affiliations

    • Copenhagen Biocenter, Department of Molecular Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark
    • ,
  • Ronald Simon

      Affiliations

    • Department of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
    • ,
  • Guido Sauter

      Affiliations

    • Department of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
    • ,
  • Martin W. Berchtold

      Affiliations

    • Copenhagen Biocenter, Department of Molecular Biology, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark
    • Corresponding Author InformationCorresponding author. Tel.: +45 35322089; fax: +45 35322128.

Received 26 June 2007 ,Revised 14 August 2007 ,Accepted 14 August 2007.

References 

  1. Chatellard-Causse C, Blot B, Cristina N, Torch S, Missotten M, Sadoul R. Alix (ALG-2-interacting protein X), a protein involved in apoptosis, binds to endophilins and induces cytoplasmic vacuolization. J. Biol. Chem. 2002;277:29108–29115
  2. Chen C, Sytkowski AJ. Apoptosis-linked gene-2 connects the Raf-1 and ASK1 signalings. Biochem. Biophys. Res. Commun. 2005;333:51–57
  3. la Cour JM, Mollerup J, Berchtold MW. ALG-2 oscillates in subcellular localization, unitemporally with calcium oscillations. Biochem. Biophys. Res. Commun. 2007;353:1063–1067
  4. la Cour JM, Mollerup J, Winding P, Tarabykina S, Sehested M, Berchtold MW. Up-regulation of ALG-2 in hepatomas and lung cancer tissue. Am. J. Pathol. 2003;163:81–89
  5. Draeby I, Woods YL, la Cour JM, Mollerup J, Bourdon JC, Berchtold MW. The calcium binding protein ALG-2 binds and stabilizes Scotin, a p53-inducible gene product localized at the endoplasmic reticulum membrane. Arch. Biochem. Biophys. 2007;467:87–94
  6. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 2001;411:494–498
  7. Jang IK, Hu R, Lacana E, D'Adamio L, Gu H. Apoptosis-linked gene 2-deficient mice exhibit normal T-cell development and function. Mol. Cell Biol. 2002;22:4094–4100
  8. Katoh K, Suzuki H, Terasawa Y, Mizuno T, Yasuda J, Shibata H, et al. The penta-EF-hand protein ALG-2 interacts directly with the ESCRT-I component TSG101, and Ca2+-dependently co-localizes to aberrant endosomes with dominant-negative AAA ATPase SKD1/Vps4B. Biochem. J. 2005;391:677–685
  9. Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, et al. Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat. Med. 1998;4:844–847
  10. Krempler A, Henry MD, Triplett AA, Wagner KU. Targeted deletion of the Tsg101 gene results in cell cycle arrest at G1/S and p53-independent cell death. J. Biol. Chem. 2002;277:43216–43223
  11. Li L, Cohen SN. Tsg101: a novel tumor susceptibility gene isolated by controlled homozygous functional knockout of allelic loci in mammalian cells. Cell. 1996;85:319–329
  12. Mahul-Mellier AL, Hemming FJ, Blot B, Fraboulet S, Sadoul R. Alix, making a link between apoptosis-linked gene-2, the endosomal sorting complexes required for transport, and neuronal death in vivo. J. Neurosci. 2006;26:542–549
  13. Maki M, Narayana SV, Hitomi K. A growing family of the Ca2+-binding proteins with five EF-hand motifs. Biochem. J. 1997;328(Pt 2):718–720
  14. Matsuo H, Chevallier J, Mayran N, Le Blanc I, Ferguson C, Faure J, et al Role of LBPA and Alix in multivesicular liposome formation and endosome organization. Science. 2004;303:531–534
  15. Missotten M, Nichols A, Rieger K, Sadoul R. Alix, a novel mouse protein undergoing calcium-dependent interaction with the apoptosis-linked-gene 2 (ALG-2) protein. Cell Death Differ. 1999;6:124–129
  16. Mollerup J, Krogh TN, Nielsen PF, Berchtold MW. Properties of the co-chaperone protein p23 erroneously attributed to ALG-2 (apoptosis-linked gene 2). FEBS Lett. 2003;555:478–482
  17. Odorizzi G. The multiple personalities of Alix. J. Cell Sci. 2006;119:3025–3032
  18. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al Towards a proteome-scale map of the human protein–protein interaction network. Nature. 2005;437:1173–1178
  19. Schraml P, Kononen J, Bubendorf L, Moch H, Bissig H, Nocito A, et al. Tissue microarrays for gene amplification surveys in many different tumor types. Clin. Cancer Res. 1999;5:1966–1975
  20. Shibata H, Suzuki H, Yoshida H, Maki M. ALG-2 directly binds Sec31A and localizes at endoplasmic reticulum exit sites in a Ca2+-dependent manner. Biochem. Biophys. Res. Commun. 2007;353:756–763
  21. Sperandio S, Poksay K, de Belle I, Lafuente MJ, Liu B, Nasir J, et al. Paraptosis: mediation by MAP kinases and inhibition by AIP-1/Alix. Cell Death Differ. 2004;11:1066–1075
  22. Strack B, Calistri A, Craig S, Popova E, Gottlinger HG. AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell. 2003;114:689–699
  23. von Schwedler UK, Stuchell M, Muller B, Ward DM, Chung HY, Morita E, et al The protein network of HIV budding. Cell. 2003;114:701–713
  24. Tarabykina S, Moller AL, Durussel I, Cox J, Berchtold MW. Two forms of the apoptosis-linked protein ALG-2 with different Ca(2+) affinities and target recognition. J. Biol. Chem. 2000;275:10514–10518
  25. Tarabykina S, Mollerup J, Winding P, Berchtold MW. ALG-2, a multifunctional calcium binding protein?. Front. Biosci. 2004;9:1817–1832
  26. Tomsig JL, Snyder SL, Creutz CE. Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif. J. Biol. Chem. 2003;278:10048–10054
  27. Trioulier Y, Torch S, Blot B, Cristina N, Chatellard-Causse C, Verna JM, et al. Alix, a protein regulating endosomal trafficking, is involved in neuronal death. J. Biol. Chem. 2004;279:2046–2052
  28. Vito P, Lacana E, D'Adamio L. Interfering with apoptosis: Ca(2+)-binding protein ALG-2 and Alzheimer's disease gene ALG-3. Science. 1996;271:521–525
  29. Vito P, Pellegrini L, Guiet C, D'Adamio L. Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction. J. Biol. Chem. 1999;274:1533–1540
  30. Wu Y, Pan S, Luo W, Lin SH, Kuang J. Hp95 promotes anoikis and inhibits tumorigenicity of HeLa cells. Oncogene. 2002;21:6801–6808
  31. Yamasaki A, Tani K, Yamamoto A, Kitamura N, Komada M. The Ca2+-binding Protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A. Mol. Biol. Cell. 2006;17:4876–4887

PII: S1574-7891(07)00066-X

doi: 10.1016/j.molonc.2007.08.002

Molecular Oncology
Volume 1, Issue 4 , Pages 431-439 , April 2008

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