Use of high density antibody arrays to validate and discover cancer serum biomarkers

Loch, Christian M.; Ramirez, Arturo B.; Liu, Yan; Sather, Cassandra L.; Delrow, Jeffrey J.; Scholler, Nathalie; Garvik, Barbara M.; Urban, Nicole D.; McIntosh, Martin W.; Lampe, Paul D.

doi:10.1016/j.molonc.2007.08.004

« Previous Next »Molecular Oncology
Volume 1, Issue 3 , Pages 313-320, December 2007

Use of high density antibody arrays to validate and discover cancer serum biomarkers

Christian M. Loch
- Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Arturo B. Ramirez
- Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Yan Liu
- Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Cassandra L. Sather
- Genomics Shared Resource, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Jeffrey J. Delrow
- Genomics Shared Resource, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Nathalie Scholler
- Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Barbara M. Garvik
- Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Nicole D. Urban
- Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Martin W. McIntosh
- Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
Paul D. Lampe
- Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA
- Corresponding author. Fred Hutchinson Cancer Research Center, Public Health Sciences, 1100 Fairview Avenue North, M5C800, Box 19024, Seattle, WA 98109, USA. Tel.: +1 206 667 4123; fax: +1 206 667 2537.

Received 18 May 2007; received in revised form 22 August 2007; accepted 23 August 2007. published online 20 September 2007.

Abstract

Perhaps the greatest barrier to translation of serum biomarker discoveries is the inability to evaluate putative biomarkers in high throughput validation studies. Here we report on the development, production, and implementation of a high-density antibody microarray used to evaluate large numbers of candidate ovarian cancer serum biomarkers. The platform was shown to be useful for evaluation of individual antibodies for comparative analysis, such as with disease classification, and biomarker validation and discovery. We demonstrate its performance by showing that known tumor markers behave as expected. We also identify several promising biomarkers from a candidate list and generate hypotheses to support new discovery studies.

Keywords: Biomarker, Antibody microarray, Serum, Ovarian, Cancer