Estradiol–estrogen receptor: A key interplay of the expression of syndecan-2 and metalloproteinase-9 in breast cancer cells

Kousidou, Olga Ch.; Berdiaki, Aikaterini; Kletsas, Dimitris; Zafiropoulos, Alexandros; Theocharis, Achilleas D.; Tzanakakis, George N.; Karamanos, Nikos K.

doi:10.1016/j.molonc.2008.06.002

« Previous Next »Molecular Oncology
Volume 2, Issue 3 , Pages 223-232, October 2008

Estradiol–estrogen receptor: A key interplay of the expression of syndecan-2 and metalloproteinase-9 in breast cancer cells

Olga Ch. Kousidou
- Laboratory of Biochemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Department of Chemistry, University of Patras, 261 10 Patras, Greece
Aikaterini Berdiaki
- Department of Histology, School of Medicine, University of Crete, Heraklion, Greece
Dimitris Kletsas
- Laboratory of Cell Proliferation and Ageing, Institute of Biology, N.C.S.R. “Demokritos”, 153 10 Athens, Greece
Alexandros Zafiropoulos
- Department of Histology, School of Medicine, University of Crete, Heraklion, Greece
Achilleas D. Theocharis
- Laboratory of Biochemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Department of Chemistry, University of Patras, 261 10 Patras, Greece
George N. Tzanakakis
- Department of Histology, School of Medicine, University of Crete, Heraklion, Greece
Nikos K. Karamanos
- Laboratory of Biochemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Department of Chemistry, University of Patras, 261 10 Patras, Greece
- Corresponding author. Tel.: +30 2610 997915; fax: +30 2610 997153.

Received 24 April 2008; received in revised form 5 June 2008; accepted 10 June 2008. published online 07 July 2008.

Abstract

Estrogens are related with the growth and development of target tissues and play a critical role in breast cancer progression. The effects of estrogens are mediated by the estrogen receptors ERα and ERβ, which are members of the nuclear steroid receptor superfamily. To date, it is not known how these hormones elicit many of their effects on extracellular matrix molecules and how these effects can be connected with ER expression. For this purpose, the effect of estradiol on ER expression as well as on proteoglycan and metalloproteinase expression was studied. The effect of E2 on extracellular matrix molecule expression has been studied using ERα suppression in breast cancer cells. Our studies using ERα-positive MCF-7 cells show that estradiol affects the expression of syndecan-2, but not of syndecan-4, through ERα. Furthermore, the ability of estradiol to affect MMP-9 and TIMP-1 expression is connected with ERα status. Together, these data demonstrate the significant role of ERα on mediating the effect of estradiol on extracellular matrix molecules.

Keywords: Proteoglycans, Metalloproteinases, Tissue inhibitors of metalloproteinases, Estradiol, Breast cancer, Estrogen receptor α

Abbreviations: E2, estradiol, PG, proteoglycan, MMP, matrix metalloproteinase, TIMP, tissue inhibitor of metalloproteinase, ECM, extracellular matrix, ER, estrogen receptor, ERE, estrogen responsive element