The CALM and CALM/AF10 interactor CATS is a marker for proliferation

Archangelo, Leticia Fröhlich; Greif, Philipp A.; Hölzel, Michael; Harasim, Thomas; Kremmer, Elisabeth; Przemeck, Gerhard K.H.; Eick, Dirk; Deshpande, Aniruddha Jayant; Buske, Christian; Hrabé de Angelis, Martin; Olalla Saad, Sara Teresinha; Bohlander, Stefan K.

doi:10.1016/j.molonc.2008.08.001

« Previous Next »Molecular Oncology
Volume 2, Issue 4 , Pages 356-367, December 2008

The CALM and CALM/AF10 interactor CATS is a marker for proliferation

Leticia Fröhlich Archangelo
- Department of Medicine III, University of Munich Hospital Grosshadern and Clinical Cooperative Group Leukemia, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
- Hematology and Hemotherapy Center, State University of Campinas (UNICAMP), Carlos Chagas 480, 13083-970 Campinas-SP, Brazil
Philipp A. Greif
- Department of Medicine III, University of Munich Hospital Grosshadern and Clinical Cooperative Group Leukemia, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
Michael Hölzel
- Institute of Clinical Molecular Biology and Tumor Genetics, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
- Present address: Division of Molecular Carcinogenesis/H2, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
Thomas Harasim
- Institute of Clinical Molecular Biology and Tumor Genetics, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
Elisabeth Kremmer
- Institute of Molecular Immunology, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
Gerhard K.H. Przemeck
- Institute of Experimental Genetics, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany
Dirk Eick
- Institute of Molecular Immunology, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
Aniruddha Jayant Deshpande
- Department of Medicine III, University of Munich Hospital Grosshadern and Clinical Cooperative Group Leukemia, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
Christian Buske
- Department of Medicine III, University of Munich Hospital Grosshadern and Clinical Cooperative Group Leukemia, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
Martin Hrabé de Angelis
- Institute of Experimental Genetics, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany
Sara Teresinha Olalla Saad
- Hematology and Hemotherapy Center, State University of Campinas (UNICAMP), Carlos Chagas 480, 13083-970 Campinas-SP, Brazil
Stefan K. Bohlander
- Department of Medicine III, University of Munich Hospital Grosshadern and Clinical Cooperative Group Leukemia, German Research Center for Environmental Health, Marchioninistrasse 25, 81377 Munich, Germany
- Corresponding author. Department of Medicine III, Grosshadern Hospital, Marchioninistrasse 15, 81377 Munich, Germany. Tel.: +49 (0)89 7099 357; fax: +49 (0)89 7095 5551.

Received 2 May 2008; received in revised form 26 August 2008; accepted 27 August 2008. published online 24 September 2008.

Abstract

The CATS protein was recently identified as a novel CALM interacting protein. CATS increases the nuclear and specifically the nucleolar localization of the leukemogenic CALM/AF10 fusion protein. We cloned and characterized the murine Cats gene. Detailed analysis of murine Cats expression during mouse embryogenesis showed an association with rapidly proliferating tissues. Interestingly, the Cats transcript is highly expressed in murine hematopoietic cells transformed by CALM/AF10. The CATS protein is highly expressed in leukemia, lymphoma and tumor cell lines but not in non-proliferating T-cells or human peripheral blood lymphocytes. CATS protein levels are cell cycle dependent and it is induced by mitogens, suggesting a role of CATS in the control of cell proliferation and possibly CALM/AF10-mediated leukemogenesis.

Keywords: CALM/AF10, Leukemia, Nucleolus, Proliferation, CATS